With an advent of technology in laboratory diagnostics

With an advent of technology in laboratory diagnostics, culture still remainsthe gold standard in identification of many organisms (Mahon, Manuselis and Lehman, 2011). This is a bit challenging because we are dealing with a stool sample where a wide range of normal flora resides. Some of this normal flora closely resembles enteric pathogens based on their colonial morphology, reactions to special and selective media as well as its biochemical reaction to specific battery of tests. With this experiment we will try to isolate, identify, differentiate and confirm enteric bacterial pathogens, such as Salmonella species, Shigella species, Yersiniaspecies, Escherichia coli O157:H7, Campylobacter species, Vibrio species,Aeromonas species and Plesiomonas species. Some laboratory are also testing for Edwardsiella tarda and Escherichia coli O104:H4 (German EnterohaemorrhagicE.coli) (ref# lab manual WDHB). Parasitic and viral pathogens have been ruled out on the stool sample submitted, as well as the Clostridium difficile and its toxin, they were all tested negative.
We will also discuss some of the virulence factors and clinical significance of organisms identified.